Why Is The Extraction Period Typically 24 Hours In China For Cytotoxicity Testing?

GB/T 16886.12-2017 recommends using a 24-hour extraction period for cytotoxicity testing with serum-containing media, which provides essential nutrients for cell growth. However, these nutrients are susceptible to time and environmental temperature changes. Currently, ISO/TC194 WG5 is conducting round-robin tests for implant cytotoxicity with 72-hour extraction followed by 24, 48, and 72-hour cell contact periods. Improvements to cytotoxicity testing will be based on international comparison results.